June 12, 2024

How to Cultivate Mycelium at Home

So you want to cultivate your own mycelium at home, huh? Well you’ve come to the right place. Growing fungi may sound complicated but with some basic equipment and a little patience, you’ll have your own mushroom spawn in no time. Mycelium, the thread-like cells that make up the mushrooms you know and love, is fascinating to watch spread and grow. And the best part is, with a few simple steps, you can become a mycelium farmer right in your own home. In this article, we’ll walk you through everything you need to get started – from sourcing mushroom spores to providing the perfect environment for your new fungal friends to flourish. Before you know it, you’ll have a bumper crop of oyster, shiitake or whatever mushrooms you choose to cultivate. So roll up your sleeves, clear some space, and let’s get ready to grow some mushrooms!

How to Cultivate Mycelium at Home
How to Cultivate Mycelium at Home

What Is Mycelium and Why Grow It?

Mycelium is the vegetative part of fungi, consisting of a mass of branching, thread-like hyphae. Growing your own edible mycelium at home, like oyster mushrooms, can be an enjoyable hobby.

Why Grow Your Own?

There are a few reasons to cultivate mycelium yourself:

  • Cost-effectiveness. Once you have the necessary equipment, you can grow pounds of mushrooms for little money. Commercial mushrooms can be pricey.
  • Know your source. You’ll know exactly what’s in your mushrooms and where they came from. No mystery additives.
  • Environmental sustainability. Growing your own reduces the resources needed to transport commercial mushrooms.

Getting Started

To get started, you’ll need a few basic supplies:

  1. Spawn (mycelium starter culture) of your desired mushroom strain. Popular edible varieties for beginners include oyster, shiitake, and lion’s mane.
  2. A container like a bucket, tub, or tray. A lid or cover is useful but not always essential.
  3. Organic hardwood sawdust, straw, corncobs or other substrate for the mycelium to colonize.
  4. A spray bottle to keep the substrate moist.
  5. A humid, sterile area with temperatures of 65-72°F.

Once you have the supplies, combine the spawn with your substrate in the container, keep humid and wait as the mycelium colonizes it. With the right conditions, you’ll have homegrown mushrooms in 1 to 3 weeks! Mycelium cultivation is a rewarding hobby, and your homemade mushrooms will be worth the effort.

· Did you know that mushrooms are as absorbent as sponges?
· Pesticides sprayed on them by farmers encompass their whole being.
· Now, you can turn to growing healthiest and the tastiest mushrooms!
· The Comprehensive Guide: “You Deserve to Eat Healthy Mushrooms! Free from Harmful Pesticides!

Choosing the Right Mycelium Strain

So you want to grow your own mycelium at home, huh? Good choice – it’s a fun and rewarding hobby. But first, you’ll need to decide which strain of mycelium you want to cultivate.

There are many types of mycelium that produce edible mushrooms, like oyster mushrooms, shiitake, and lion’s mane. These varieties are delicious and nutritious, making them great for home cultivation. Oyster mushrooms, in particular, are easy for beginners and fast-growing.

For something more exotic, you might consider strains that produce psychedelic mushrooms, though these may be illegal to grow where you live, so check your local laws first. Some psilocybin-containing strains, like Golden Teacher and B+, tend to be more potent.

Finding Spores

Once you’ve chosen a strain, you’ll need to obtain spores or live culture to get started. You can buy spores legally online for most edible strains. Live cultures, also called ‘spawn’, are also available for some types. Spores are more affordable but take longer since you have to germinate them first. Spawn is ready to inoculate right away but costs more.

Either way, look for reputable suppliers that provide detailed information about each strain so you know exactly what you’re getting. And be extremely cautious if attempting to cultivate any psychedelic varieties, as there are legal risks involved.

With the right spores or spawn in hand, some basic equipment, and a little patience, you’ll be growing mycelium in no time. But choosing a strain you genuinely want to cultivate will make the whole process that much more rewarding.

Getting the Growing Conditions Right

To cultivate mycelium at home, providing the right growing conditions is key. Mycelium requires humidity, temperature control, nutrients, and substrate to thrive.


Mycelium needs humidity around 95-100% to grow, so maintaining moisture is critical. You’ll want to keep your mycelium in an enclosed container, like a plastic bin with lid or an aquarium with a secured top. Add moisture to the container using a spray bottle to mist the inside, or place damp paper towels in the bottom. Check on your mycelium at least once a day and mist with water if the humidity drops.


Most mycelium species grow best in warm conditions, around 70 to 80 F. Place your mycelium container in a spot away from vents or drafts. A closet, cabinet or box can help regulate temperature. Use a heating pad on a low setting if additional warmth is needed.


Mycelium feeds on nutrients to grow, so providing the proper food source, known as a substrate, is essential. Substrates like rice, grain, wood or straw give mycelium carbohydrates to consume. Add the substrate to your container before introducing mycelium spores or culture. Bury about an inch of substrate under more substrate or casing layer.

Casing layer

A casing layer placed over the substrate helps maintain moisture and provides more nutrients. Materials like coir, vermiculite, peat moss or shredded paper work well. The casing should be a few inches deep, damp but not soggy.

With the right humidity, temperature, nutrients and casing layer, your mycelium will spread threads throughout the substrate and casing, developing into a fully colonized block or patch. Monitor your mycelium regularly and make any needed adjustments to ensure optimal growing conditions. In a week or two, you’ll have homegrown mycelium to use in various applications!

Read more:
How Do Mushroom Grow Kits Work
How to Attract Pollinators for Plants
Backyard Urban Gardening: Cultivating Nature in the Concrete Jungle

Step-by-Step Guide to Inoculating and Incubating

Once you have your spores or culture and necessary equipment, you’re ready to inoculate and incubate your mycelium. This step-by-step guide will walk you through the process.

Selecting a Substrate

Choose a nutritious substrate, like grain (rye, wheat, oats), sawdust, or straw. Grain and sawdust work well for beginners. Sterilize the substrate in an autoclave or pressure canner according to package directions. Let cool completely.

Preparing the Containers

Use sealable containers with gas exchange filters or holes, like polypropylene bags, glass jars with lids, or food-grade plastic containers. Sterilize containers and any tools before inoculating.

Inoculating the Substrate

Work in a still air box or laminar flow hood. Add the culture or spores to the substrate using a sterile syringe. For spores, inject 1-2 cc per bag or jar. For liquid cultures, use 3-5 cc.

Sealing and Labeling the Containers

Seal each container and label with the date of inoculation and strain. Place containers in a temperature-controlled environment.

Incubating the Mycelium

Incubate for 7-30 days around 65-75°F. Check containers regularly. Growth will appear as white, ropey strands. Gently shake grain bags/jars to distribute the growth. Once fully colonized, the substrate should become solid white.

Consolidating the Mycelium

Let the mycelium rest for 1 week after full colonization. This allows the mycelium to strengthen before fruiting. You now have spawn that can be used to inoculate logs, cardboard, or bulk substrates to produce mushrooms. Or, you can fruit the spawn directly.

To summarize, be meticulous in your sterile technique, choose a suitable substrate and container, inoculate with spores or culture, seal and label, incubate until colonized, then consolidate before fruiting or expanding to bulk substrates. With practice, you’ll be cultivating mycelium with ease! Let me know if you have any other questions.

· Did you know that mushrooms are as absorbent as sponges?
· Pesticides sprayed on them by farmers encompass their whole being.
· Now, you can turn to growing healthiest and the tastiest mushrooms!
· The Comprehensive Guide: “You Deserve to Eat Healthy Mushrooms! Free from Harmful Pesticides!

Harvesting, Storing and Using Your Homegrown Mycelium

Once your mycelium has fully colonized the substrate, it’s time for the rewarding part—harvesting and using your homegrown fungi! The harvesting process is simple but important to get right.


When you notice the substrate is covered in a network of fine, white threads, your mycelium is ready to harvest. Gently brush away any loose substrate from the surface of the block. Use a knife, scalpel or sharp blade to cut wedge-shaped pieces from the edge of the block. Twist or cut to release the pieces.

Handle the mycelium carefully since the strands are delicate. Rinse the pieces briefly in cold water to remove any remaining debris. Gently pat dry with a paper towel or lint-free cloth.


Store your harvested mycelium in an airtight container, like a ziplock bag or plastic container with a lid. Keep refrigerated for up to 1 week. The mycelium can also be dehydrated for long term storage. Using a dehydrator or oven on a low setting, dehydrate the pieces until cracker dry. This can take 6 to 12 hours. Store the dehydrated mycelium in an airtight jar or bag. When ready to use, simply rehydrate in warm water.


Add the fresh or rehydrated mycelium to dishes like omelets, stir fries, and pasta for an umami boost. You can also use it as a meat substitute in dishes like mushroom burgers or mushroom “scallops”. Powdered mycelium works well as a supplement or natural flavor enhancer. Add 1/2 teaspoon at a time to sauces, gravies, and soups.

Homegrown mycelium is a rewarding ingredient to cook with. Start with a small amount, around 1/2 cup, until you get used to the earthy, savory flavor. Mix in with other ingredients or add to your favorite recipes for an easy way to enjoy the fruits of your fungal labor! With some experimenting, you’ll be creating amazing mushroom-inspired meals in no time.


Whether you want to create your own mushroom farm or just experiment with an unusual hobby, cultivating mycelium at home is a rewarding process. Now that you’ve learned the basics, you have everything you need to get started. Grab some spores or cultures, prepare your substrate, monitor conditions carefully, and in no time you’ll have a thriving colony producing mushrooms. Sure, there may be some trial and error at first, but don’t get discouraged. With regular maintenance and care for your fungi friends, you’ll be harvesting homegrown mushrooms in just a few short weeks. Who knew science could be this fun? Now get out there and spread the spores – the world of mycology awaits!